We evaluated 17 women with normal cervix and 45 women with invasive cervical carcinoma to study the association of S-phase fraction by flow cytometry and then umber of Nucleolar Organizer Regions (NORs) by silver colloid technique with the
clinical
outcome of the cervical cnacer (age, clinical stage, tumor size, depth of invasion, lymph node metastasis, 5-year disease free survival rate) using formalin fixed paraffin embedded tissues from January, 1985 to December, 1986.
We classified each two groups according to the mean numbers of AgNORs/nucleus, 1.5(group 1<1.5, group II≥1.5) and the S-phase fraction, 19.1%(group I<19.1, group II≥19.1).
@ES The results were as follows;
@EN 1. The S-phase fraction and the mean number of AgNORs in cervical carcinoma were significantly higher than those in normal cervix.
2. the S-phase fraction or the numbers of AgNORs were not significantly associated with most of the prognostic factors. But the numbers of AgNORs were significantly higher in patients with lymphnode metastasis than those without lymph node
metastasis.
3. There was no significant association between lymph node metastasis and the combination of the preonostic factors and the proliferative activity (S-phase fraction, numbers of AgNOks).
4. The specificity of flow cytometric analysis for the prediction of lymph node metastasis was relatively high but the sensitivity was low. And the sensitivity of the number of AgNORs for the prediction of lymph node metastasis was high. In
combination
with flow cytometry an dthe numbers of AgNORs, the accuracy was relatively high(73.7%).
5. there was no significant difference in 5-year disease free survival rates between each two groups in invasive cervical carcinoma.
So we concluded that the numbers of AgNORs and S-phase fraction were significantly higher in cervical carcinoma than in normal cervix, but they may not be useful as the prognostic factor in cervical carcinoma.
|